Tissue culture technique plays a significant role in the growth, morphogenesis and development of tissue protocols through the supplementation of Media having all nutrients under aseptic condition. The role of tissue culture on crops is an important siblings done since a decades. Agriculture is a term which is utilized for different types of crop and livestock production, aquaculture, fisheries, and forestry for food and non-food products. Since olden days, Agriculture seems to rise since human civilization and farming has a strong relationship for food surpluses that enabled people to live in cities.Plant tissue culture (PTC) has promising role in making disease resistant variety of crops since 20th century. The technique is highly reliable in taping the economic potentials of medicinal and crop plants. In this Research, four types of seeds of Oryzae sativa, Triticum aestivum, Zea maize and Sorghum bicolor were taken and allow to germinate on filter paper moisten with nutrient solution like macronutrient, micronutrient and Vitamin solution for 2minutes. After 2 minutes, the different types of crops seeds spread over the filter paper moistened with nutrient solution in three petriplates. A filter paper was used to cover the seeds and petriplates were kept at three individual places. In this Experiment, Istpetriplate was kept under light condition, Second kept at darkroom and 3rdpetriplate kept at open Environment. The temperature was maintained at 22 to 250C. After 48 hour of observation, the seeds seem to be germinated. The seeds were kept at dark rooms seem to germinate more rapidly in comparison to seeds kept at light and Environment condition. After observation, the germinated seeds inoculated in plant growth regulators (Auxin, Gibberlin, Cytokinin and IBA) supplement Agar plus Basal Media. After one week of culture, the seedling measure 8cm and transfer to field for their proper growth. It was found that Auxin seems to plays a significant impact on growth and development of Triticum aestivum and Oryzae sativa in comparison to and Zea maize. On the other hand, though the growth and development was observed in other plant growth regulators like Gibberlin, Cytokinin and IBA but seems to be very less active on growth and morphogenesis than Auxin Agar supplemented MS Media.
The objective of this research was to summarize a depth idea about the fruitful requirements in the tissue culture of different crop varieties which have positively contribute to the biotechnological breeding programs. Thus, plant tissue culture technique empowers good candidates of plant species to be rescued and proliferated in large number to produce disease resistant varieties of different types of crops